Troshkova G.P. 1
Kostina N.E. 1
Protsenko M.A. 1
Skarnovich M.A. 1
1 The Federal Budget Institution of Science “The State Research Center of Virology and Biotechnology Vector”
The article is devoted to the development of technology for production of dry extract from the mushroom Fomes fomentarius for use it to create effective drugs for the prevention and treatment of viral infections. The various methods of biologically active substances extraction from the mushroom FOMES FOMENTARIUS (percolation, maceration and fractional maceration) using various concentrations ethyl alcohol of have been described. The optimal values of technological parameters of the process of obtaining a dry extract from the mushroom Fomes fomentarius has been determined. The maximum content of extractives (5,6 ± 0,7) % found in the extracts, which were obtained using an alcohol solution with a concentration of 70%. (optimum temperature of the extraction process - 55-60 ° C, particles of mushroom ranging in size from 50 to 500 microns). The qualitative and quantitative analysis of biologically active substances contained in the dry extract has been performed, the presence of various biologically active substances such as proteins and polysaccharides, triterpene saponins, coumarins and phenolic compounds has been established. The leading group of biologically active compounds for further standardization of the extract has been revealed. It has been showed, that the protein content in the dried extract of the fruiting body of mushroom Fomes fomentarius ranged from 7.0 to 8.4% and polysaccharides from 53.2 to 68.2%.