Scientific journal
Balanced diet, nutritional supplements and biostimulants
ISSN 2414-1054
ISSN 2414-1054
DEVELOPMENT OF PROCEDURES FOR QUALITY CONTROL OF A NEW BIOACTIVE SUBSTANCE NA-CPAH WITH NEUROTROPIC ACTIVITY
Makarova E.A. 1
Sidullina S.A. 1
Semina I.I. 1
Tarasova R.I. 2
Moustafin R.I. 1
1 Kazan State Medical University
2 Kazan State Technological University
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We have studied the main physicochemical properties of a new bioactive substances Na-CPAH, which belongs to a class of phosphorylacetohydrazides, with neurotropic activity. Based on these properties, we have developed methods of determining the qualitative and quantitative content. Oxidation-reduction reactions of Na-CPAH with known oxidants were suggested by high-sensitivity reaction with potassium permanganate. Furthermore, there was shown the necessity to use a complex of chemical and physicochemical methods to verify the authenticity of Na-CPAH: qualitative reaction on functional groups, parameters of IR - and UV-absorption spectra. We studied its’ UV and IR absorption spectra, highlighted the characteristic bands of the major functional groups; and suggested the methodology of determining the authenticity of Na-CPAH by the IR absorption spectrum. The optimal conditions for spectrometric determination of Na-CPAH in substance were found. The method was developed for the quantitative analysis of the substance Na-CPAH of UV-spectrometry; The optimal conditions for the quantitative determination were suggested and it was found that concentration can be calculated by the index of absorption, as well as sucrose standard sample or calibration curve. The relative error in determination is not more than ± 0,72%.