Ryazanova T.K. 1
1 Samara State Medical University
We have done pharmacognostic study of bilberry shoots dried and fruits fresh and dried (Vaccinium myrtillus L.). The biologically active substances from bilberry fruits (cyanidin-3-glucoside) and bilberry shoots (quercetin-3-xylopyranoside) were isolated by using of liquid column chromathography method. Methods of qualitative analysis there were developed for identifi cation plant material by using of thin-layer chromatography and UV/Vis spectroscopy. According to the physical and chemical properties of isolated compounds spectrophotometric methods of the determination of total anthocyanins in the fruits (calculated on cyanidin-3-glucoside chloride) and total fl avonoids in shoots (calculated on rutin) there were developed. The relative degree of the determination of the total anthocyanins in fruits dried in developed method with confi dence probability 0,95 is no more than ± 3,52 %; in fruits fresh ± 4,54 %. The relative degree of the determination of the total fl avonoids in shoots in developed method with confi dence probability 0,95 is no more than ± 3,56 %.The developed methods of analysis were included in projects for pharmacopoeia specifi cations for fresh and dried bilberry fruits and dried bilberry shoots.