Bokova L.M. 1
Sultygova Z.K. 1
Bokova M.M. 1
Balaeva F.A. 1
Inarkieva Z.I. 1
1 FGBOU VPO «Ingush State University»
Defined molecular weight distribution of the protein fractions of cereals and fl our by planar gel permeation chromatography (PGPH). Investigation of protein fractions with different solubility corn PGPH method showed that the amount of protein fractions depends not only on the molecular weight, but also on the pH of the medium used eluent. Explore a wide range of values of pH 4,0 to pH 10,0. To distinguish the solvent front and the rate of motion are used stained proteins of natural origin, hemoglobin, cytochrome and «C» as well as proteins with a fluorescent label. Qualitative analysis of cereals carried out molecular weight determinations separated by silica gel plate different in nature (extraction in various solvents) protein complexes. The technique of using the optimum pH for the medium further fractionation eluent water and salt-soluble proteins.