Evseeva O.S. 1
Andreeva O.A. 1
Oganesyan E.T. 1
Aroyan M.V. 1
1 Pyatigorsk Medical and Pharmaceutical Institute
The individual flavanone was extracted from the peel of C. maxima. Using quantitative acidic flavanone hydrolysis in the hydrolyzate after neutralization D-glucose and L-rhamnose were detected by chromatographic method. On the basis of results of paper chromatography (composition of mobile phase: 15 % acetic acid & butanol : acetic acid : water (4:1:5), UV-, IR-, NMR 1H- spectroscopy, melting point the aglycone was identified as 5,7,4’-trihydroxyflavanone (naringenin), which is a yellow needle crystals free soluble in ethanol (melting point 250 оС). In the Zinc-hydrochloric acid reduction test alcoholic solution of the aglycone becomes magenta color. The assay spectrophotometric method of flavanoids in C. maxima peel by was developed (as to hesperidin, using calibration curve). Flavanoids content in the peel of C. maxima was 6,59 ± 0,1217 %.